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期刊论文 3

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2022 1

2016 1

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软件定义网络；安全；多控制器；分布式规则存储 1

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Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients

《医学前沿（英文）》 doi: 10.1007/s11684-023-0997-7

摘要： Primary ciliary dyskinesia (PCD) is a highly heterogeneous recessive inherited disorder. FAP54, the homolog of CFAP54 in Chlamydomonas reinhardtii, was previously demonstrated as the C1d projection of the central microtubule apparatus of flagella. A Cfap54 knockout mouse model was then reported to have PCD-relevant phenotypes. Through whole-exome sequencing, compound heterozygous variants c.2649_2657delinC (p. E883Dfs*47) and c.7312_7313insCGCAGGCTGAATTCTTGG (p. T2438delinsTQAEFLA) in a new suspected PCD-relevant gene, CFAP54, were identified in an individual with PCD. Two missense variants, c.4112A>C (p. E1371A) and c.6559C>T (p. P2187S), in CFAP54 were detected in another unrelated patient. In this study, a minigene assay was conducted on the frameshift mutation showing a reduction in mRNA expression. In addition, a CFAP54 in-frame variant knock-in mouse model was established, which recapitulated the typical symptoms of PCD, including hydrocephalus, infertility, and mucus accumulation in nasal sinuses. Correspondingly, two missense variants were deleterious, with a dramatic reduction in mRNA abundance from bronchial tissue and sperm. The identification of PCD-causing variants of CFAP54 in two unrelated patients with PCD for the first time provides strong supportive evidence that CFAP54 is a new PCD-causing gene. This study further helps expand the disease-associated gene spectrum and improve genetic testing for PCD diagnosis in the future.

关键词： primary ciliary dyskinesia     CFAP54     cilia    

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Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth

《医学前沿（英文）》 2022年 第16卷 第6期   页码 873-882 doi: 10.1007/s11684-022-0925-2

摘要： Tumor growth is an angiogenesis-dependent process and accompanied by the formation of hypoxic areas. Tumstatin is a tumor-specific angiogenesis inhibitor that suppresses the proliferation and induces the apoptosis of tumorous vascular endothelial cells. VNP20009, an attenuated Salmonella typhimurium strain, preferentially accumulates in the hypoxic areas of solid tumors. In this study, a novel Salmonella-mediated targeted expression system of tumstatin (VNP-Tum5) was developed under the control of the hypoxia-induced J23100 promoter to obtain anti-tumor efficacy in mice. Treatment with VNP-Tum5 effectively suppressed tumor growth and prolonged survival in the mouse model of B16F10 melanoma. VNP-Tum5 exhibited a higher efficacy in inhibiting the proliferation and inducing the necrosis and apoptosis of B16F10 cells in vitro and in vivo compared with VNP (control). VNP-Tum5 significantly inhibited the proliferation and migration of mouse umbilical vascular endothelial cells to impede angiogenesis. VNP-Tum5 downregulated the expression of anti-vascular endothelial growth factor A, platelet endothelial cell adhesion molecule-1, phosphorylated phosphoinositide 3 kinase, and phosphorylated protein kinase B and upregulated the expression of cleaved-caspase 3 in tumor tissues. This study is the first to use tumstatin-transformed VNP20009 as a tumor-targeted system for treatment of melanoma by combining anti-tumor and anti-angiogenic effects.

关键词： Salmonella VNP20009     tumstatin     B16F10     melanoma     apoptosis     angiogenesis    

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一种安全、高性能的软件定义网络多控制器体系结构 Article

Huan-zhao WANG,Peng ZHANG,Lei XIONG,Xin LIU,Cheng-chen HU

《信息与电子工程前沿（英文）》 2016年 第17卷 第7期   页码 634-646 doi: 10.1631/FITEE.1500321

摘要： 控制器在软件定义网络（software-defined networking）中扮演着至关重要的角色。然而现有的SDN控制器体系结构存在单点故障隐患，即控制器资源容易被洪泛的数据流请求耗尽。此外，由于网络应用和控制器之间复杂的交换行为，数据流建立的时延也比较大。为了解决以上SDN控制器的安全和性能问题，本文提出一种名为分布式数据存储（distributed rule store，DRS）的SDN多控制器体系结构。在DRS体系结构中，控制器将网络应用预计算的流规则进行缓存，并把这些规则分布式存储在不同的控制器实例上。每个控制器仅存储其中的一部分规则，并周期性地检查其他控制器中规则的完整性。来自交换机的请求会被分配到不同的控制器进行处理，因此针对控制器的资源耗尽攻击可以被缓解。同时，当单个控制器上的规则被恶意篡改后，DRS可以及时发现并且修复被篡改的规则。本文基于Floodlight控制器对DRS进行了实现，并通过大量模拟实验对其性能进行评价。实验结果表明DRS可以有效保证分布式规则存储的一致性，且相对于已有的ONOS和Floodlight控制器，数据流建立的时间更短、吞吐量更大。

关键词： 软件定义网络；安全；多控制器；分布式规则存储    

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标题 作者 时间 类型 操作

Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients

期刊论文

Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth

期刊论文

一种安全、高性能的软件定义网络多控制器体系结构

Huan-zhao WANG,Peng ZHANG,Lei XIONG,Xin LIU,Cheng-chen HU

期刊论文